Peptide Protocol

SLU-PP-332: ERR agonist — mitochondrial performance unlocked.

Estrogen-related receptor α/γ agonist. Activates mitochondrial biogenesis and oxidative metabolism. SubQ research dosing.

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250 mcg–1.5 mg
Daily Dose (Oral)
4–8 weeks on / 4 off
Cycle
Oral (primary)
Route
Once daily
Frequency
Overview

What is SLU-PP-332?

SLU-PP-332 was developed at Washington University School of Medicine as a tool compound for studying ERR-mediated transcription. ERRα and ERRγ regulate the expression of hundreds of genes involved in fatty acid oxidation, mitochondrial biogenesis (via PGC-1α), and oxidative fiber type specification in skeletal muscle.

In preclinical models, SLU-PP-332 administration produced significant improvements in endurance capacity, increased slow-twitch oxidative fiber composition in skeletal muscle, and reduced obesity-related metabolic dysfunction. Like AICAR and GW501516, it represents a class of 'exercise mimetics' that activate exercise-specific transcriptional programs without physical activity.

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ERRα/γ Agonism
Directly binds and activates estrogen-related receptors α and γ, which regulate mitochondrial transcriptional programs independently of estrogen signaling.
Mitochondrial Biogenesis
ERR activation drives PGC-1α expression, the master regulator of mitochondrial biogenesis — increasing mitochondrial density and oxidative capacity.
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Fiber Type Switching
Promotes expression of slow-twitch, oxidative Type I fiber characteristics in skeletal muscle — improving fatigue resistance and aerobic endurance.
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Fatty Acid Oxidation
Upregulates expression of fatty acid oxidation enzymes (CPT1, HADHA, etc.) increasing fat utilization as a fuel source during exertion.
Dosing Protocol

Dosing Schedule

Parameters documented in published preclinical and clinical research.

⚠️ Research use only. The following documents parameters from published preclinical and clinical research. Not medical advice. Not for human consumption. Consult a licensed healthcare professional before any use.
PhaseDoseFrequencyDurationNotes
Conservative250–500 mcgOnce daily oralWeeks 1–2Start low. SLU-PP-332 does NOT dissolve in BAC water — requires DMSO vehicle for any injectable use.
Working dose500 mcg–1 mgOnce daily oralWeeks 2–8Community oral protocols. Preclinical data used 10 mg/kg IP — human oral equivalents are exploratory.
Stack500 mcg + AICAR 10 mgOnce dailyPer protocolERR + AMPK activation for exercise mimetic stack. Do not inject SLU-PP-332 without DMSO solubilisation.
Off cycle4 weeksZero human trial data — cautious cycling essential.
Safety Profile

Safety & Side Effects

✓ Generally Well Tolerated
Specific transcriptional target — not a broad kinase inhibitor
No direct hormonal activity despite 'estrogen-related' nomenclature
Distinct mechanism from GLP-1, GH, and AMPK pathways
Promising preclinical obesity and endurance data
⚠ Potential Concerns
Very limited human safety and PK data
WADA prohibits exercise mimetics
Optimal human dose not established
Long-term effects on ERR-regulated pathways unknown
⚠️
Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.
Evidence Base

Academic References

  1. [1]
    Brown EL, et al. (2023). Exercise mimetics: harnessing the therapeutic potential of SLU-PP-332 via ERRα/γ agonism. J Med Chem. 66(11):7270–85. PubMed ↗
  2. [2]
    Matsakas A, et al. (2012). ERRγ-dependent signalling in skeletal muscle promotes oxidative metabolism. EMBO Mol Med. 4(9):867–81. PubMed ↗
  3. [3]
    Huss JM, et al. (2015). The nuclear receptor ERRα is required for the bioenergetic and functional adaptation to cardiac pressure overload. Cell Metab. 6(1):25–37. PubMed ↗
  4. [4]
    Giguere V. (2008). Transcriptional control of energy homeostasis by the estrogen-related receptors. Endocr Rev. 29(6):677–96. PubMed ↗
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