How much BAC water do I add to a Humanin vial?

Humanin is typically supplied in 5–10 mg vials. Use 1–2 mL of BAC water. The calculator provides exact draw volumes for any target dose.

How should reconstituted Humanin be stored?

Store at 2–8°C and use within 28 days. Lyophilized powder is stable at -20°C.

Humanin Dosing Calculator & Protocol

What is Humanin?

Humanin was discovered in 2001 by Hashimoto and colleagues while screening mitochondrial genome sequences for factors that could rescue neurons from Alzheimer's disease-related apoptosis. It is one of the first identified mitochondria-derived peptides (MDPs) — a class of small peptides encoded in the mitochondrial genome that function as endocrine-like signals.

Circulating humanin levels are positively correlated with health and longevity — higher levels are found in centenarians' offspring, and lower levels are associated with metabolic syndrome, cardiovascular disease, and cognitive decline. It acts on multiple receptor complexes to promote cell survival, improve insulin sensitivity, reduce inflammation, and protect against oxidative stress.

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Multi-Receptor Signaling

Acts on FPRL1, tripartite CNTFR/WSX-1/gp130 complex, and intracellular targets to activate JAK2-STAT3, PI3K-AKT, and MAPK survival pathways.

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Anti-Apoptotic

Directly binds and neutralizes pro-apoptotic proteins (BAX, tBID), preventing mitochondrial outer membrane permeabilization and cytochrome c release.

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Insulin Sensitization

Activates AMPK and improves hepatic and skeletal muscle insulin signaling, reducing blood glucose and improving metabolic parameters.

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Neuroprotection

Originally discovered for its ability to rescue neurons from Alzheimer's-related and ischemic apoptosis — crosses blood-brain barrier via active transport.

Dosing Schedule

Parameters documented in published preclinical and clinical research.

⚠️ Research use only. The following documents parameters from published preclinical and clinical research. Not medical advice. Not for human consumption. Consult a licensed healthcare professional before any use.

Phase	Dose	Frequency	Duration	Notes
Research	0.5 mg	2× weekly SubQ	Weeks 1–4	Conservative start based on mouse-scaled pharmacology.
Working dose	1–2 mg	2–3× weekly	Weeks 4–12	Most research protocols use 2 mg/week in divided SubQ doses.
HNG analog	0.1–0.5 mg	2× weekly	Per protocol	HNG (S14G-Humanin) is ~1000× more potent — dose accordingly.
Off cycle	—	—	4–8 weeks	Limited human data — periodic assessment recommended.

Phase

Dose

Frequency

Duration

Notes

Research

0.5 mg

2× weekly SubQ

Weeks 1–4

Conservative start based on mouse-scaled pharmacology.

Working dose

1–2 mg

2–3× weekly

Weeks 4–12

Most research protocols use 2 mg/week in divided SubQ doses.

HNG analog

0.1–0.5 mg

2× weekly

Per protocol

HNG (S14G-Humanin) is ~1000× more potent — dose accordingly.

Off cycle

—

4–8 weeks

Limited human data — periodic assessment recommended.

Safety & Side Effects

✓ Generally Well Tolerated

Endogenous peptide — naturally produced in all human tissues

Positive correlation with longevity in human studies

Multiple cytoprotective mechanisms documented

No significant adverse effects in preclinical safety studies

⚠ Potential Concerns

Primarily preclinical data — formal human clinical trials limited

Optimal human dosing not established

Short plasma half-life — frequent dosing needed

HNG analog requires dose reduction due to dramatically increased potency

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Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.

Academic References

[1]

Hashimoto Y, et al. (2001). A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Aβ. Proc Natl Acad Sci USA. 98(11):6336–41. PubMed ↗

[2]

Muzumdar RH, et al. (2009). Acute humanin therapy attenuates myocardial ischemia and reperfusion injury in mice. Arterioscler Thromb Vasc Biol. 30(10):1940–8. PubMed ↗

[3]

Lee C, et al. (2015). The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. 21(3):443–54. PubMed ↗

[4]

Sreekumar PG, et al. (2019). The emerging role of senescence in ocular disease. Oxid Med Cell Longev. 2019:1438951. PubMed ↗

Humanin: the mitochondrial hormone — cell survival signaling.

Humanin
Dosage Protocol

What is Humanin?

Dosing Schedule

Safety & Side Effects

Academic References

Humanin: the mitochondrial hormone — cell survival signaling.

HumaninDosage Protocol

What is Humanin?

Dosing Schedule

Safety & Side Effects

Academic References

Humanin
Dosage Protocol