peppercalc.com · research use only
Peptide Protocol

GHK-Cu: Copper peptide for skin, collagen, and longevity research.

Glycine-histidine-lysine copper complex. Protocol for skin regeneration, wound healing, and longevity research.

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peppercalc.com · research use only
1–2 mg/day
Daily Dose
8–12 weeks
Cycle
SubQ / Topical
Route
Once daily
Frequency
Overview

What is GHK-Cu?

GHK-Cu (Gly-His-Lys-Cu2+) is a tripeptide that occurs naturally in the body, with plasma concentrations declining significantly with age. Research has demonstrated its ability to stimulate collagen, fibronectin, and decorin synthesis; activate wound-healing pathways; and modulate a broad array of gene expression — including genes related to inflammation, nervous system function, and cancer suppression.

Its copper-chelating ability may also contribute to antioxidant activity, while its role as a growth signal appears to operate via fibroblast activation. The compound is studied both for systemic longevity mechanisms (subcutaneous) and local skin regeneration (topical application or intradermal injection).

Collagen Synthesis
Stimulates collagen I, III, and fibronectin production in fibroblasts — key mechanism for skin repair and wound healing.
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Gene Modulation
Shown to modulate over 31% of human genes in research; affects inflammation, apoptosis, and tissue remodelling pathways.
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Antioxidant
Copper complex contributes to antioxidant defence; may reduce oxidative stress in aged tissues.
Longevity Signalling
Declining GHK-Cu plasma levels with age have been correlated with tissue degeneration in research. Administration of exogenous GHK-Cu is being studied for its effects on longevity-related signalling pathways.
Dosing Protocol

Dosing Schedule

Parameters documented in published preclinical and clinical research.

⚠️ Research use only. The following documents parameters from published preclinical and clinical research. Not medical advice. Not for human consumption. Consult a licensed healthcare professional before any use.
PhaseDoseFrequencyDurationNotes
Conservative0.5–1 mgOnce daily8 weeksStarting dose for systemic research. GHK-Cu requires extended use — effects build over 8–12 weeks.
Standard typical1–2 mgOnce daily8–12 weeksMost research falls in this range. Minimum 8 weeks for meaningful collagen outcomes.
Topical / IntradermalVariableDaily as neededOngoingFor skin-specific research. Concentration depends on formulation.
Off cycle4 weeksRest between systemic cycles.
Safety Profile

Safety & Side Effects

✓ Generally Well Tolerated
Naturally occurring in human body
Excellent topical safety profile
No androgenic effects
Broad research base with favourable safety signals
⚠ Potential Concerns
Systemic subcutaneous use has less data than topical
Potential skin discolouration at high intradermal doses
Copper accumulation theoretical concern with extended use
Injection site reactions possible
⚠️
Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.
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