How much BAC water do I add to a 5-Amino-1MQ vial?

Use the calculator with your specific vial size and target dose to find the optimal reconstitution volume.

How should reconstituted 5-Amino-1MQ be stored?

Store at 2–8°C and use within 28 days. Lyophilized powder is stable at -20°C.

5-Amino-1MQ Dosing Calculator & Protocol

What is 5-Amino-1MQ?

5-Amino-1-methylquinolinium (5-Amino-1MQ) is a cell-permeable NNMT inhibitor developed at Weill Cornell Medicine. NNMT is overexpressed in obese adipose tissue and acts as a metabolic sink for methyl groups — its inhibition restores SAM levels, activates downstream AMPK and SIRT1 pathways, and suppresses adipocyte differentiation.

Preclinical data in diet-induced obese mice show significant reduction in fat mass and body weight without changes in food intake or lean mass, suggesting it targets fat specifically. Research suggests it may also enhance NAD+ metabolism and energy expenditure. It can be administered orally or subcutaneously, with most research protocols favoring oral capsule formulations for convenience.

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NNMT Inhibition

Blocks nicotinamide N-methyltransferase, preventing methylation of nicotinamide and restoring elevated SAM levels in adipose tissue.

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AMPK Activation

Elevated SAM ratios activate AMPK signaling, the master metabolic regulator that promotes fatty acid oxidation and suppresses lipogenesis.

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Adipogenesis Suppression

Reduces differentiation of pre-adipocytes into mature fat cells, limiting new fat storage capacity and reducing adipose tissue expansion.

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NAD+ Pathway Support

NNMT inhibition reduces competitive consumption of NAD+ precursors, potentially enhancing NAD+ availability for sirtuin activation and mitochondrial function.

Dosing Schedule

Parameters documented in published preclinical and clinical research.

⚠️ Research use only. The following documents parameters from published preclinical and clinical research. Not medical advice. Not for human consumption. Consult a licensed healthcare professional before any use.

Phase	Dose	Frequency	Duration	Notes
Start	50 mg	Once daily	Days 1–14	Oral capsule. Assess GI tolerance and energy response.
Working dose	50–100 mg	Once or twice daily	Weeks 2–8	Most research protocols use 50 mg 2× daily or 100 mg once daily.
Stack	50 mg	With NAD+	Ongoing	Often stacked with NAD+ for synergistic metabolic and energy effects.
Off cycle	—	—	2 weeks	Cycle off periodically to reassess response.

Phase

Dose

Frequency

Duration

Notes

Start

50 mg

Once daily

Days 1–14

Oral capsule. Assess GI tolerance and energy response.

Working dose

50–100 mg

Once or twice daily

Weeks 2–8

Most research protocols use 50 mg 2× daily or 100 mg once daily.

Stack

50 mg

With NAD+

Ongoing

Often stacked with NAD+ for synergistic metabolic and energy effects.

Off cycle

—

2 weeks

Cycle off periodically to reassess response.

Safety & Side Effects

✓ Generally Well Tolerated

Oral administration — no injection required

Targets adipose-specific metabolism — limited systemic effects

No direct catecholamine stimulation

Research shows fat-specific effects without lean mass loss

⚠ Potential Concerns

Primarily preclinical data — limited human trial evidence

Long-term safety profile not established

May interact with methylation pathways broadly

Quality and purity variation between suppliers

⚠️

Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.

Academic References

[1]

Kannt A, et al. (2018). Association of nicotinamide-N-methyltransferase mRNA expression in human adipose tissue with insulin resistance. PLOS ONE. 10(8):e0135421. PubMed ↗

[2]

Brachs S, et al. (2016). Inhibition of citrate cotransporter Slc13a5/mINDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease. Mol Metab. 5(11):1072–82. PubMed ↗

[3]

Neelakantan H, et al. (2019). Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochem Pharmacol. 163:481–92. PubMed ↗

[4]

Hong S, et al. (2015). Nicotinamide N-methyltransferase regulates hepatic nutrient metabolism through Sirt1 protein stabilization. Nat Med. 21(8):887–94. PubMed ↗

5-Amino-1MQ: NNMT inhibition — epigenetic fat burning.

5-Amino-1MQ
Dosage Protocol

What is 5-Amino-1MQ?

Dosing Schedule

Safety & Side Effects

Academic References

5-Amino-1MQ: NNMT inhibition — epigenetic fat burning.

5-Amino-1MQDosage Protocol

What is 5-Amino-1MQ?

Dosing Schedule

Safety & Side Effects

Academic References

5-Amino-1MQ
Dosage Protocol