Peptide Protocol

AOD-9604: GH fragment — fat loss without the IGF-1.

C-terminal fragment of hGH (176-191). Stimulates lipolysis without raising blood glucose or IGF-1. SubQ once daily fasted.

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300–500 mcg
Daily Dose
12–16 weeks
Cycle
SubQ
Route
Once daily (fasted)
Frequency
Overview

What is AOD-9604?

AOD-9604 is the hGH fragment 176-191 — the specific region of the GH molecule responsible for fat metabolism regulation. By isolating this fragment, researchers eliminated the muscle-building and insulin-antagonizing effects of full GH while preserving its lipolytic actions. AOD-9604 activates β3-adrenergic receptors in adipose tissue to stimulate lipolysis.

Phase 2 clinical trials for obesity demonstrated significant visceral fat reduction with daily AOD-9604 administration. Importantly, it does not stimulate IGF-1 production, does not cause insulin resistance, and does not elevate blood glucose — key safety advantages over full GH administration for metabolic research. It is administered fasted in the morning to maximize lipolytic effect.

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Lipolysis Activation
Activates β3-adrenergic receptors in adipose tissue, triggering cAMP-mediated lipase activity and fatty acid release from fat stores.
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IGF-1 Neutral
Unlike full GH, AOD-9604 does not stimulate IGF-1 production, avoiding the anabolic and growth-promoting effects of the full GH molecule.
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Glucose-Safe
Does not antagonize insulin action or elevate blood glucose — a key safety advantage for longer metabolic research protocols.
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Fat Formation Inhibition
Also inhibits fat differentiation and lipogenesis, reducing new fat deposition in addition to mobilizing existing fat stores.
Dosing Protocol

Dosing Schedule

Parameters documented in published preclinical and clinical research.

⚠️ Research use only. The following documents parameters from published preclinical and clinical research. Not medical advice. Not for human consumption. Consult a licensed healthcare professional before any use.
PhaseDoseFrequencyDurationNotes
Start300 mcgOnce daily (fasted)Weeks 1–4Morning SubQ on empty stomach. Can also be administered intranasally.
Working dose300–500 mcgOnce daily (fasted)Weeks 4–16Wait 30–60 min after injection before eating for best effect.
Advanced500–1000 mcgOnce dailyPer protocolHigher doses used in some trials — diminishing returns reported above 500 mcg.
Off cycle4–8 weeksCycle off to reassess metabolic response.
Safety Profile

Safety & Side Effects

✓ Generally Well Tolerated
Phase 2 clinical trial data for visceral fat reduction
No IGF-1 stimulation — avoids anabolic side effects
No effect on blood glucose or insulin sensitivity
Generally well tolerated in clinical trials
⚠ Potential Concerns
Not FDA-approved; clinical program discontinued after Phase 3
Intranasal formulation has inconsistent bioavailability
Long-term safety data limited
Subcutaneous administration site reactions possible
⚠️
Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.
Evidence Base

Academic References

  1. [1]
    Heffernan M, et al. (2001). The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice. Mol Cell Endocrinol. 184(1-2):137–46. PubMed ↗
  2. [2]
    Ng FM, et al. (2000). Metabolic studies of a synthetic lipolytic domain (AOD9604) of human growth hormone. Horm Res. 53(6):274–8. PubMed ↗
  3. [3]
    Heffernan MA, et al. (2000). Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone fragment 176-191. Int J Obes. 25(10):1442–9. PubMed ↗
  4. [4]
    Stier H, et al. (2013). Safety and tolerability of the GH fragment AOD9604 in healthy adults. Clin Exp Pharmacol Physiol. 40(5):352–6. PubMed ↗
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