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Cycle
Overview
What is TB-500?
Thymosin Beta-4 is one of the most abundant intracellular peptides in the body. TB-500, the synthetic fragment corresponding to the actin-binding domain (Ac-SDKP), retains many of TB4's key bioactivities. It promotes cell migration, proliferation, and differentiation — mechanisms central to tissue repair across muscle, tendon, skin, and cardiac tissue.
Unlike BPC-157, which tends to work locally near the injection site, TB-500 has demonstrated systemic activity in animal models. This means it may act on tissues throughout the body regardless of injection location. Researchers often combine it with BPC-157 (Wolverine Stack) for complementary local and systemic repair.
🔬Actin Sequestration
Binds G-actin to regulate cell shape, motility, and migration — the primary mechanism behind its wound-healing activity.
🩸Angiogenesis
Promotes blood vessel formation and endothelial cell migration, improving nutrient supply to damaged tissues.
🌐Systemic Action
Unlike local peptides, TB-500 may act at injury sites distant from the injection point via systemic circulation.
🛡️Anti-inflammatory
Downregulates inflammatory cytokines and reduces local inflammatory response at injury sites.
Reconstitution
Reconstitution Calculator
Enter your vial size and BAC water volume to calculate your exact concentration, syringe units, and number of doses per vial.
Interactive Calculator
Dose Planner for TB-500
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Step-by-step
How to reconstitute
1
Gather supplies
Lyophilised peptide vial, bacteriostatic water, a drawing syringe (1–3 mL), U-100 insulin syringes for dosing, and alcohol swabs. Work on a clean surface.
2
Swab and dry
Wipe stoppers on both vials with alcohol swabs. Wait 10–15 seconds for full evaporation before piercing. Never blow or touch after swabbing.
3
Add BAC water slowly
Draw BAC water into your syringe, insert into peptide vial, and inject slowly down the inside glass wall — never directly onto the powder cake.
4
Swirl gently — do not shake
Roll the vial between your palms until powder fully dissolves. Solution should be clear and colourless. Shaking degrades the peptide.
💡 If still cloudy after 2 minutes, gently swirl again and wait. Discard if permanent cloudiness or particulates appear.
5
Label and refrigerate
Label with compound name, concentration, and reconstitution date. Store at 2–8 °C. Most reconstituted peptides are stable for 4–6 weeks refrigerated.
Dosing Protocol
Dosing Schedule
Research-derived dosing ranges. Always begin conservatively to assess tolerance.
| Phase | Dose | Frequency | Duration | Notes |
|---|
| Loading phase weeks 1–6 | 10–20 mg/week | Split into 2 injections | 6 weeks | Higher dose to saturate tissues and initiate healing cascade. |
| Maintenance phase | 5–10 mg/week | 1–2 injections | Ongoing as needed | Reduced dose to sustain repair and reduce inflammation. |
| Acute injury protocol | 20 mg/week | Split into 2 injections | 4–8 weeks | For active injuries requiring accelerated intervention. |
| Off cycle | — | — | 4–8 weeks | Rest period between extended cycles. |
🌶️
Use the PepperCalc calculatorEnter your exact vial size and BAC water above to get your precise syringe units for each dose. No guesswork.
Administration
Injection Sites
🎯
Subcutaneous
Most common route. Inject into subcutaneous fat of abdomen, thigh, or near injury.
💪
Intramuscular
Some protocols use IM injection for potentially faster absorption. Glute or thigh preferred.
🌐
Site Flexibility
Due to systemic action, injection site may be less critical than with locally-acting peptides.
Safety Profile
Safety & Side Effects
✓ Generally Well Tolerated
Broad safety data in animal models
No reported hormonal suppression
Systemic action without systemic hormonal effects
Generally well tolerated across dose ranges studied
⚠ Potential Concerns
Fatigue or tiredness (especially early in loading phase)
Mild injection site irritation
Limited long-term human data
WADA prohibited (S2) in competitive sports
⚠️
Research use onlyThis page is an educational reference. None of this constitutes medical advice. Consult a qualified professional before any use. All compounds are for research purposes only.
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